In Vitro Propagation of Croton (Codiaeum Variegatum Gold Dust.) using Nodal Explants from Field Grown Plants

In Vitro Propagation of Croton (Codiaeum Variegatum Gold Dust.) using Nodal Explants from Field Grown Plants

Document Type : Research papers

Abstract

This study was carried out in the tissue culture laboratory , the Faculty of Agriculture, Saba basha, Alexandria University, Egypt during the period from 2012 to 2014 . An efficient and reliable protocol for in vitro propagation of croton (Codiaeum variegatum Gold Dust.) was optimized. However, nodal explants from field grown of Croton (Codiaeum variegatum Gold Dust. ) were used during in vitro culture study for induction of multiple shoots. Nodal explants were inoculated on various initiation or establishment media with different combinations of NAA and BA and the neoformed shoots were cultured on proliferation (multiplication) media for the development of multiple shoots, and the elongation media to elongate of the neoformed shoot. The subsequent elongated shoots were rooted, and acclimatized ex vitro, successfully. The best medium for shoot initiation was WPM medium supplemented with 1.0 mg/l BA. The favourable medium for multiplication was the tested medium augmented with 2.0 mg/l BA and 0.50 mg/l NAA. In addition, the most effective medium for elongation was the used medium enriched with 1.0 mg/l NAA. Furthermore, th in vitro shoots showed healthy root development when the tested medium was supplemented with combination of 2.0 mg/l IBA and 0.50 mg/l NAA (rooting stage).The combination of sand: peat moss (4:1) was used as substratum for the hardening of the in vitro plantlets, as a potting mix, was the best suited mix for the acclimatization of plantlets.

Keywords

References

Ahn ,Y.J., L. Vang , A.Thomas , M.C. Keon and G. Q. Chen. 2007. High-frequency plant regeneration through adventitious shoot formation in castor (Ricinus communis L.). In Vitro Cell. Develop. Biology-Plant, 43: 9-15.

-Ashish, S. and R.A.Sharma . 2011.Micropropagation of Croton Bonplandium Ball.Inter. Res . J. Pharm (IRJP) , 2(10):82-86.

Awal, S. M. A.; M. R. A. Alam and M. N. U. Hasan.2005. In vitro propagation of pointed gound (Trichosanthes dioica Roxb.) from shoot tips. Biotech.,4(3): 221-224.

Bhattacharya,P., S. Dev, and B.S.Bhattacharya.1990. Rapid mass propagation of Chysanthemum morifolium by callus derived from stem and leaf explants.Pl.Cell Rep.9:439-442.

Bhot,M., S. Naphade ,J. Varghese and N. Chandra . 2010. In vitro culture studies in three Varieties of Codiaeum variegatum (L.) Blume using node explants from fieldgrowth plants J. Cell and Tiss. Res., 10(3) 2439-2444

Bingtao, L.I,Q. Huaxing , M. Jin-shuang , Z. Hua, G. Michael ,G. Hans-Joachim ,E. Stefan Dressler ,P. Hoffmann ,LJ. Gillespie ,M. Vorontsova and GD. McPherson . 2008. Flora of China. http://www.efloras.org, dated 22nd September, 11: 163.

Chen, Y.Z., X. D. He,P.Y. Jiang and C.M. Wang. 1985. In vitro propagation ofchrysanthemum leaves.J. Jin. Agric.,6(4):33-36.

Chen J.J.,CA. Robinson , R.D. Caldwell and D.B. McConnell . 2000.Waste composts as component of container substrates for rooting foliage plant cuttings Proc. Fla. State. Hort .Soc., 112:272-274.

Chitra P. and V. Madhusoodanan . 2005. Influence of auxins in direct in vitro morphogenesis of Euphorbia nivulia, a lectinaceous medicinal plant.

In Vitro Cell. Develop. Biol –Pl., 41: 314-319.

Dai-Bisheng (2007). Effect of carbendazim plus thiram and triadimefon plus ethylicin on the survival rate of three kind softwood cuttings.Huazhong Shifan Daxue Xuebao (ZiranKexueBan), 41(1):111-116.

Davies,P.J.1995.PlantHormones:Physiology,BiochemistryandMolecularBiology.Dordrecht:Kluwer.833p.

Deepa D. N. and A. Shanthi 2013. Propagating crotons from leaves. African J. o Agri. Res., 8(26) :3473-3475.

Gomez, K. and A. A.Gomez. 1984. Statistical procedures for Agricultural Research (2 ed.). An International Rice Research Institute Bok. A Wiley Interscience Publisher, New York.

George,E.F.andP.D.Sherrington.1984.Plant propagation by tissue culture.Exegeetic Ltd.,Basingtoke,U.K.709 P.

George,E.F.,M.A.Hall and G.J.D.Klerk.2008.Plant Propagation by Tissue Culture.3^rd Edition.Springer.

Komalavalli,N.and M.V.Rao.2000. in vitro micro-propagation of Gymnemam Slyvestre.Amultipurpose medicinal plant.Pl.Cell,Tiss.Org.Cul.,61:97-105.

Kupchan, S.M., I. Uchida, A.R. Branfman, R.C. Dailey and B.Y. Fei. 1976. Antileukemic principles isolated from Euphorbiaceae plants. Sci., 191:571-572.

Liu,H.W.,H.Zhang,Z.F.Ma andY.Liang.1994.Fast breeding of groundcover chrysanthemum.J.North.Fores.Univ., 22(1):31-35.

Lloyd, G. and B. McCown. 1980. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. Proc. Intl. Pl. Prop. Soc., 30: 421-427.

Maciel, A.M., A.C. Pinto, S.N. Brabo and M.N. Silva. 1998. Terpenoids from Croton cajura. Phytochem., 49: 823-826.

Martins, A.P., L.R. Salgueiro, M.J. Conclaves, R. Vila, F. Tomi, T. Adzet, A.P. Cunha, S. Canigueral and J. Casanova. 2002. Antimicrobial activity and chemical composition of bark oil of Croton stellulifer. Planta Medi., 66: 647-652.

Martin K.P.,C. Sundarakumari ;M. Chitra ,P.V. Madhusoodhanan . 2005. Influence of auxins in direct in vitro morphogenesis of Euphorbia nivulia, a lectinaceous medicinal plant, In Vitro Cell. Develop. Biol.-Pl., 41: 314-319.

Mulabagal, V. and H.S. Tsay. 2004. Plant Cell Cultures: An alternative and efficient source for the production of biologically important secondary metabolites. Int. J. of App. Sci. and Eng., 2(1): 29-48.

Munshi,M.K.,L.Hakim,M.R.Islam and G.Ahmed.2004.in vitro clonal propagation of Banyan(Ficus benghalensis L.) through axillary bud culture. Int. J. Agric. Biol., 6(2): 321-323.

Murashige,T.1974.Plant propagation through tissue culture.Ann.Rev.Plant Physiol., 25:135-166

Nasib, A . , K. Ali and S.Khan. 2008.In vitro propagation of croton (Codiaeum variegatum).Pak. J.Bot., 40 (1):99-104

Ogunwenmo K.O., O.A. Idowu , C. Innocent , E.B. Esan and O.A.Oyelana .2007. Cultivars of Codiaeum variegatum (L.) Blume (Euphorbiaceae) show variability in phytochemical and cytological characteristics. Afr. J. Biotechnol., 6(20):2400-2405.

Orlikowska, T., I. Sabata and D. Kucharska. 2000. The effect of leaf and shoot tip removal and explant orientation on adillary shoot proliferation ofCodiaeum variegatum Blume var. pictum Muell. Arg. Cv. Excellent. Sci.Horti., 85(1-2): 103-111.

Puebla, P., J.L. Lopez, M. Guerrero, R. Carron, M.L. Martin, L.S. Roman and A.S. Feliciano. 2003.Neo-clerodane diterpenoids from Croton schiedeanus. Phytochem., 62: 551-554.

Radice,S.2010. Micropropagation of Codiaeum variegatum(L.)Blume and regeneration induction via Adventitious Buds and Somatic Embryogenesis.

.In:Jain,S.M & S.J.Ochatt(eds.,)Protocols for In VitroPropagation of Ornamental Plants, Methods in Molec. Biol.,589: 187-195

Rajani, H. and S.S.Patil.2009.In vitro response of different explants types onshoot and root development of Ginger.ISHS. Acta Hort.829:VI Inter.Symp. In Vitro Cult.Hort.Breeding.

Rajesh, K. K, S. R. Murthy, and T. Pullaiah .2009. Euphorbiaceae- a cultural review on plant tissue culture [Euphorblaceae– UNA Revision Crltica Sobre Cultivo DeeteJldoos]. Trop. & Subtrop. Agroeco., 10: 313-335.

Raven,P.H., R.F.Evert and S.E.Eichhorn.1992.Biology of Plants. New York: Worth. Pp.545-572.

Rout, G.R., A. Mohapatra , S. Mohan Jain . 2006. Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects. Biotech. Adv.,24: 531-560.

Salisbury,F.B. and C.W.Ross.1992.Plant Physiology. Belmont, A:Wadsworth. pp.357-407.

Sana, S., S. Mathew and R.S. Krishnapriy .2012. Organogenesis and Somatic Embryogenesis in various Cultivars of Codieaum Variegatum (L.) Global Advanced Res. J. Biotec., 1(3)pp. 040-047.

Sarker, R. H. and I.Shaheen.2001.In Vitro propagation of chrysanthemum (Chrysanthemum morifolium Ramat) through allus.Pl.Tiss.Cult.,11(1):85-91.

Shibata, W., F. Murai, T. Akiyama, M. Siriphol, E. Matsunaga and H. Morimot. 1996.Micropropagation of Croton sublyratus Kurz; a tropical tree of medicinal importance. Plant Cell Rep., 16: 147-152.

Silva, B. O. da, A.C.F. Amaral, J.L.P.Ferreira, L.J.M. Santiago, and R.P.Louro .2013. Micropropagation and in vitro production of secondary metabolites of Croton floribundus Spreng. In Vitro Cell. Develop. Biol. Pl., 49(3):366-372.

Smart, L. 2008. EFB530 Plant Physiology, Cytokinins and cell division, EFB530 Plant Physiology-Syllabus with lecture notes - spring 2008.

http://www.esf.edu/efb/course/EFB530/EFB530Syllabus.htm.

Steel, R. G. D.,J.H. Torrie and D. A. Dickie. 1997.Principles and procedures of statistics-a biometric approach. Thired edition. McGraw-Hill Publishing Company. Toronto.

Tillmann M.A.A., C. Cavariani , Z. Piana and K. Minami . 1994.Comparacao enter diversos substrates no enraizamento de estacas de croton (Codieaum variegatum L.).Sci Agric, 51 (1):17-20

Tomas, I. A.1987. Hormonal regulation of apical dominance. In: P. J. Davis (ed.). Plant hormones and their role in plant growth anddevelopment. Mortinus Nijoff Publishers.Dordrecht, PP. 397-410.

Trigiano, R.N. and D.J. Gray. 2000. Editors, Plant Tissue Culture Concepts and Laboratory Exercises 2 nd Edition, CRC Press,Boca Raton,430 pp.

Vijaya, N. , G. Satyanarayana, J. Prakashand and R.L.M.Pierik.1991.Effect of culture media and growth regulators on in vitro propagation of rose .Hortic. New Tech. Appl Proce. Inter. Sem. New Frontiers in Hort., organized by Indo –American Hybrid Seeds,Bangalore,Ind.,Nov.25-28,209-214.

Waseem, K., M. S. Jilani, M. S. Khan, M. Kiran and G. khan. 2011.Efficient in vitro regeneration of chrysanthemum(Chrysanthemum morifolium L.) plantlets from nodal segments.Afri.J.Biotechn.,10(8):1477-1484.

Wilkins,M.B.1989.Advanced plant physiology.The Bath Press,Avon,13-15.

Yamuna, P.,S.Das, T.B. Jha;andS.Jha.1995.Regeneration and multiplication of shoots in Glochidion multiloculaire.Muell-Arg.,Journal of Herbs,Spices and Medicinal Plants,3:67-74