Nassar, M., Fathalla Rehab, I., Kandil, E., El-Banna, A., Mohmed Ibrahim, K. (2017). Response of Some New Hybrids of Maize to Mineral and Organic Fertilization in Reclaimed Soil. Journal of the Advances in Agricultural Researches, 22(2), 360-374. doi: 10.21608/jalexu.2017.180469
Mohamed Ahmed AbdElGawad Nassar; Ibrahim Fathalla Rehab; Essam Esmael Kandil; Ali El-Banna; Kamal AbdElMonem Mohmed Ibrahim. "Response of Some New Hybrids of Maize to Mineral and Organic Fertilization in Reclaimed Soil". Journal of the Advances in Agricultural Researches, 22, 2, 2017, 360-374. doi: 10.21608/jalexu.2017.180469
Nassar, M., Fathalla Rehab, I., Kandil, E., El-Banna, A., Mohmed Ibrahim, K. (2017). 'Response of Some New Hybrids of Maize to Mineral and Organic Fertilization in Reclaimed Soil', Journal of the Advances in Agricultural Researches, 22(2), pp. 360-374. doi: 10.21608/jalexu.2017.180469
Nassar, M., Fathalla Rehab, I., Kandil, E., El-Banna, A., Mohmed Ibrahim, K. Response of Some New Hybrids of Maize to Mineral and Organic Fertilization in Reclaimed Soil. Journal of the Advances in Agricultural Researches, 2017; 22(2): 360-374. doi: 10.21608/jalexu.2017.180469
Response of Some New Hybrids of Maize to Mineral and Organic Fertilization in Reclaimed Soil
1Plant production Department, The Faculty of Agriculture (Saba Basha), Alexandria University, Egypt
2Faculty of Agriculture saba basha, Alexandria University
Abstract
Two field experiments were conducted at North of El Tahrir, El- Behira, Egypt, during 2015 and 2016 seasons, to study the effect of mineral fertilization and organic manure on three maize hybrids. Split plot design with three replications was used, where the main plots contain combination of mineral fertilization (M) + organic manure (OM) i.e., (100 % M,75% M+ 25% OM, 50% M +50% OM, 75% M +25%OM and 100% OM), meanwhile the three maize hybrids (SC.2031, SC.2030 and TWC1100) were allocated in the sub plots. Plot size was 10.50 m2 (3 m x 3.5 m) having 5 ridges of 3 m in length and 0.7 m in width. The obtained results revealed that combined application of 75% mineral + 25% organic manure) affected significantly increase in plant height (cm), ear length, number grains/row, number of grains/ear, 100 - grain weight, grain, straw, and biological yield (kg/fed.), and harvest index %. The maize hybrid SC. 2031 recorded the highest means values of above mentioned characters. Fertilizing maize hybrid SC2031 by 75 % mineral + 25% organic manure gave the highest grain yield and its components under this study.
Maize (Zea mays L.) is one of the most widely grown cereals in the world. Also, it is important summer grain crops in Egypt. There is wide gap between consumption and local production. This gap could be narrowed through use the high yielding hybrids as well as use optimum organic and mineral fertilization.
Organic farming is an excellent soil amendment which gave a balance of nutrients, while contributing valuable organic material to the soil. Soil microorganisms improved water holding capacity, soil structure for pH buffering and the organic complexing of nutrients, making them available for plant uptake. It is well documented that the incorporation of organic manure into the soil is increasingly important because it improves soil fertility and increased crop yield (Singh, 2001).
Significant increase in plant height, dry matter, yields and NPK contents with the application of nitrogen fertilizers. Maize growth, dry matter, yields and NPK contents improved significantly when treated compost were added, but it was still below the fertilizer treatments. On comparative basis, maize response was better with FYM (Memon et al., 2012). Maize yield and its components such as number of cobs/plant, cob length, number of grains/cob, and 100- grain weight were maximum when plots were fertilized at 100 kg/ha., as urea + poultry manure at rate 12.98 t/ha. (Nasim et al., 2012). Application of nitrogen at the rate of 120 kg N/ha applied as 50% urea + 50% FYM can appreciably enhance the growth and yield indices of maize crop. Bilal et al. (2016) concluded that integrated fertilization of nitrogen sources as 50% FYM and 50% urea at the rate of 120 kg N/ha resulted in higher yield and yield related traits of maize crop and is therefore recommended for achieving higher yield and yield attributes of maize crop.
The soil with organic manure continually applied had lower bulk density and higher porosity values, porous and buffering capacities (Edmeades, 2003).Proper application of organic and inorganic fertilizers can increase the activities of soil microorganisms and enzymes and soil available nutrient contents (He and Li, 2004; Saha et al., 2008).
100 grain weight, yield and yield components and N, P and K of maize plants were increased significantly by increasing the level of nitrogen up to 140 kg N/fed (Siam et al., 2008) Nitrogen levels had significant effects on yield and yield components in maize hybrids (Sharifi and Taghizadeh, 2009).The level of nitrogen fertilization differentiated the chlorophyll content expressed in SPAD units (Szulc and Hubert, 2010). 100 grain weight, grain yield and straw yield increased with increasing the rate of N fertilizer up to 120 kg N/ha. Treatment of 120 kg N/fed in four doses as 40, 20, 20 and 20 % added after 14, 28, 48, and 56 days after sowing (DAS), recorded higher values of 100 grain weight, straw and grain yield of maize (El-Agrodi et al., 2011).
N and P levels significantly increased the studied parameters of corn plants as compared with control. The highest values were obtained by using 120 kg N+ 35 kg P2 O5/ fed., followed by 80 kg N+ 25 kg P2 O5/ fed., and 40 kg N+15 kg P2 O5/fed (Ibrahim and Hala, 2007).
The Single Cross 3084 significantly surpassed other hybrids in number of grains/ear, ear weight, grain weight/ear and grain yield/fed. It was noticed that the maximum values of plant and 100-grain weight, ear weight, grain yield/fed were obtained by application of 120 kg N/fed as a mineral form. Whereas, no significant difference was noticed between 90 kg N min.+30 kg N org./fed and 120 kg N min/fed on most characters studied in both seasons (El-Gizawy and Salem, 2010).
The objective of this investigation was to study the impact of organic manure for new maize hybrids as sown reclaimed soil on yield and its components and their interaction.
MATERIALS AND METHODS
Two field experiments were carried out to study the effect of mineral fertilization and organic manure on yield, yield components of three maize hybrids and their interaction. Field experiments were conducted at North of El Tahrir, El-Beheara Governorate, Egypt, during the two successive seasons 2015 and 2016.
A split plot with three replications was used whereas main plots were combination of mineral fertilization (M) and organic manure (OM) namely; 100% mineral fertilization (120 kg N/fed, 24 kg P2O5/fed and 24 kg K2O/fed.), 75% mineral (90 kg N/fed,18 kg P2O5/fed and 18 K2O kg K2O /fed) + 25% organic manure (2.5 ton/fed), 50% mineral (60 kg N/fed, 12 kg P/fed and 12 kg K2O/fed) + 50% organic manure (5 ton/fed.), 25% mineral (30 kg N/fed, 6 kg P/fed. and 6 kg K2O /fed.) + 75% organic manure (7.5 ton/fed) and 100% organic manure (10 ton/fed) while the three maize hybrids namely; Single cross 2031 (S.C. 2031), single cross 2030 (SC. 2030) and three way cross (TWC.1100) were distributed in subplot. Plot size was 10.50 m2 (3 m x 3.5 m) having 5 ridges of 3 m in length and 0.7 m in width. Two grains were hand planted in each hill. Plant spacing 25 spacing 25 cm between plants.
Some physical and chemical characteristics of the studied soil before sowing are presented in Table (1) which were determined according to Klute (1986).
Mineral fertilization consists of nitrogen as in form of ammonium nitrate (NH4NO3 33.5 %), phosphorus as calcium super phosphate (15.5 % P2O5) and potassium as potassium sulphate (48 % K2O).
Organic manure (cow manure) at the one rate was added (10 tons/fed) at the soil preparation before planting in both seasons analysis of organic manure is presented in Table (2).
Table (1). Some physical and chemical properties of the experimental soil sites during 2015 and 2016 seasons
Soil parameter
Season
2014
2015
Particle size distribution %
Sand
Silt
Clay
Textural class
58.40
11.00
30.60
Sandy loam
57.10
11.8
31.10
Sandy loam
Chemical properties:
pH (1:1) (soil: water suspension)
EC (1:1) (soil: water extract), dS/m
Soluble cations (1:2) (meq/l)
K+
Ca++
Mg++
Na++
7.92
3.70
0.46
2.60
3.50
12.10
8.00
3.80
0.48
2.50
3.40
12.50
Soluble anions (1:2) (meq/l)
HCO-3
CL
SO-4
6.00
8.00
4.40
6.60
8.20
4.00
Calcium carbonate, %
21.25
19.85
Total nitrogen, %
0.75
0.85
Available P (mg/kg)
4.42
4.55
Organic matter, %
1.47
1.45
Table (2).Composition of organic manure (cow manure)
Determination
Cow manure
Unit
Moisture
13.70
%
Organic matter (%)
62.93
%
Total N
1.20
%
Total P
1.40
%
Total K
1.10
%
pH(1:5)
8.35
-
EC (1:5)
1.40
dS/m
C:N
30.4:1
-
Planting dates were on 25th May in 2015 and 2016 cropping seasons, respectively.
Phosphorus fertilizer was applied before planting. Field was hand thinned before the first irrigation to one plant/hill. The experimental units were hand hoed twice for controlling weeds before the first and second irrigations. N fertilizer was applied in two equal doses, the first dose was before the first irrigation and the second one was before the second irrigation during cropping seasons. Potassium sulphate (48 % K2O) was added before the first irrigation.
All other agricultural treatments for maize plants were done as recommended by the Egyptian Ministry of Agriculture. Preceding crop was wheat in both seasons.
Plant height and yield traits were determined at harvest time after 120 days from planting, three medium ridges (36 plants), which were taken from each sub plot in which grain yield was determined on the basis of 15.5 % moisture and the following data were recorded: Plant height (cm), ear length (cm), number of grains /row, number of grains /ear, 100 - grain weight (g), grain yield, straw yield, biological yield (kg/fed), and harvest index (%).
Data were subjected to analysis of variance according to Gomez and Gomez (1984). All statistical analysis was performed using analysis of variance technique by means of CoStat computer software package (CoStat, Ver. 6.311., 2005). The least significant differences (LSD at 0.05) used to compare the treatment means.
RESULTS AND DISCUSSION
Data in Table (3) revealed the effect of combination of mineral and organic fertilization and their interaction on plant height (cm), ear length (cm) and number of grains/row for the three maize hybrids during 2015 and 2016 seasons.
With regard to the effect of mineral fertilization and organic manure affected significantly in plant height, ear length and number of grains/row of the maize in both seasons, whereas application of 75 % mineral fertilization and 25% organic manure recorded the highest mean values for these traits followed by application of 100% mineral fertilization, respectively, during two seasons (Table 3). He and Li (2004) indicated that combined application of organic and inorganic fertilizers can increase the activities of soil and available nutrient content which increased growth and yield attributes of plants. Also, Kandil (2004) revealed that using organic fertilizer caused significant increases in plant height, ear length and number of kernels/row of maize.
Also, Table (3) revealed that there was significant difference among the three maize hybrids on plant attributes namely; plant height, ear length number of grains/row in both growing seasons. Where, the SC. 2031 achieved the tallest plant height, ear length and the highest mean value of number of grains/row. Variations in grain number might be due to differences in genetic potential of maize hybrids. On the other side there was no significant difference among the three maize hybrids in the first season. These results are in harmony with Kandil (2013) who indicated that there was significant difference among maize hybrids in plant height (cm), ear length (cm) and number grains/row.
The data in Table (3) indicated that there was significant interaction between the combination of mineral + organic manure on plant height (cm), ear length (cm) and number of grains/row. Whereas the highest mean values for plant height, ear length and number of grains/row were obtained from SC. 2031 when fertilization 75% mineral + 25% organic manure in seasons 2015 and 2016.Meanwhile the lowest one recorded with TWC 1100 + 100% organic manure during two seasons. On the other hand there is no significant interaction between two factors on ear length in the first season.
Table (3). Average of plant attributes for three maize hybrids as affected by mineral fertilization (M), organic manure (OM) and their interaction during 2015 and 2016 seasons
Attributes
Fertilizer treatments (F)
Season 2015
Season 2016
Maize hybrid (H)
Average (F)
LSD at 0.05
Maize hybrid (H)
Average
(C)
LSD at 0.05
SC.
2031
SC.
2030
TWC
1100
F
H
F x H
SC.
2031
SC.
2030
TWC
1100
F
H
FxH
Plant height
(cm)
100 % Mineral
346.7
326.7
283.2
318.9a
11.4
6.8
15.4
336.3
328.7
316.0
327.0a
11.9
7.0
15.6
75 % M +25% OM
346.7
326.7
298.3
323.9a
339.0
330.0
314.0
327.7a
50 M + 50 OM
336.7
326.7
303.3
322.2a
324.7
315.7
300.3
313.6b
25 M + 75 OM
286.7
299.3
303.7
296.6b
309.7
310.0
311.7
310.5b
100% OM
305.3
321.0
283.3
303.2b
317.3
310.0
278.0
301.8b
Average (H)
324.4a
320.1a
294.4b
325.4a
318.9a
304.0b
Ear length
(cm)
100 % Mineral
23.4
25.2
23.4
24.0a
1.3
1.1
ns
22.5
24.6
23.5
23.5b
0.10
0.16
0.22
75 % M +25% OM
25.0
25.4
23.3
24.6a
24.5
24.9
23.4
24.3a
50 M + 50 OM
22.2
22.2
21.7
22.0b
22.1
21.0
22.0
21.7c
25 M + 75 OM
21.0
18.9
16.8
18.9c
19.7
21.0
18.1
19.6d
100% OM
19.3
18.3
17.8
18.5c
20.1
19.0
18.0
19.0e
Average (H)
22.2a
22.0a
20.6b
21.8b
22.1a
21.0c
Number of grains/row
100 % Mineral
49.3
49.3
53.3
50.6a
2.3
ns
3.9
52.0
49.0
49.7
50.2a
0.46
0.42
3.9
75 % M +25% OM
52.0
48.7
47.3
49.3ab
53.7
50.3
48.0
50.7a
50 M + 50 OM
47.7
51.0
46.0
48.2b
46.0
45.7
50.7
47.5b
25 M + 75 OM
43.3
40.0
46.7
43.3c
44.0
41.0
42.0
42.3c
100% OM
41.3
42.7
40.7
41.6c
44.0
40.7
40.0
41.6d
Average (H)
46.7
46.3
46.8
47.9a
45.3c
46.1b
- Mean values in the same column/row marked with the same letters are not significantly different at 0.05 level of probability.
- ns.: not significant difference at 0.05 level of probability according to LSD.
Table (4) showed the effect of mineral and organic fertilization and their interaction on number of grains/ear, 100- grain weight (g) and grain yield (kg/fed.) for the three maize hybrids in 2015 and 2016 seasons.
With regard to the effect of mineral fertilization and organic manure, it is significantly affected number of grains/ear, 100- grain weight and grain yield (kg/fed.) of maize in both seasons, whereas 75 % mineral and 25% organic manure recorded the highest mean values for number of grains/ear (in the second season only), 100- grain weight and grain yield in two seasons (Table 4).In this respect Kandil (2004) revealed that using organic fertilizer caused significant increases in yield and its components of maize. These findings agreed with Siam et al. (2008), Szulc and Hubert (2010), Memon et al. (2012), and Bilal et al. (2016).
Table (4) reported that there was significant difference among the three hybrids on number of grains/ear, 100- grain weight and grain yield (kg/fed.) in both cropping seasons. Where, the SC. 2031 gave the highest number of grain/ear and grain yield in two seasons. While the highest value of 100- grain weight recorded with TWC1100 in both season. While Sc.203 recorded the lowest one in 2015 and 2016. Variations in grain number might be due to differences in genetic potential of maize hybrids. These findings are in agreement with El-Gizawy and Salem (2010), Ahmed (2011), and Kandil (2013) who indicated that there was significant difference among maize hybrids in 100- grain weight, grain yield and number grains/ear.
The interaction between the combination of mineral + organic manure on number of grains/ear, 100- grain weight and grain yield (kg/fed.) were significant (Table 4). Whereas the highest values for number of grains/ear in both season, and grain yield (kg/fed.) in the first season only were obtained from SC. 2031 when fertilization 75% mineral + 25% organic manure in seasons 2015 and 2016.on the other wiseTWC1100 + 75% mineral + 25% organic recorded the heaviest 100+ grain weight in two seasons. Meanwhile the lowest one recorded with TWC 1100 + 100% organic manure during two seasons. On the other hand there is no significant interaction between two factors on grain yield/fed in the second season.
Table (4). Average of plant attributes for maize hybrids as affected by mineral fertilization (M),organic manure (OM) and their interaction during 2015 and 2016 seasons
Attributes
Fertilizer treatments (F)
Season 2015
Season 2016
Maize hybrid (H)
Average (F)
LSD at 0.05
Maize hybrid (H)
Average (F)
LSD at 0.05
SC.
2031
SC.
2030
TWC
1100
F
H
F x H
SC.
2031
SC.
2030
TWC
1100
F
H
F x H
Number of grains/ear
100 % Mineral
690.7
592.0
746.7
676.5a
32.3
23.7
52.9
728.0
588.0
695.3
670.4a
6.1
5.4
12.2
75 % M +25% OM
728.0
584.0
662.7
658.2ab
751.3
604.0
672.0
675.8a
50 M + 50 OM
667.3
612.0
6.44.0
639.7b
644.0
548.0
709.3
633.8b
25 M + 75 OM
606.7
480.0
653.3
580.0c
616.0
492.0
588.0
565.3c
100% OM
578.7
512.0
569.3
553.3c
616.0
488.0
560.0
554.7d
Average (H)
654.3a
556.0b
658.0a
671.1a
544.0c
644.9b
100 grain weight (g)
100 % Mineral
42.6
42.4
45.2
43.4b
0.14
0.15
0.34
43.4
43.5
45.5
44.1b
0.17
0.16
0.37
75 % M +25% OM
44.2
44.7
47.1
45.3a
46.0
44.7
47.7
46.1a
50 M + 50 OM
41.8
41.7
47.1
43.5c
40.2
41.9
41.2
41.1c
25 M + 75 OM
41.1
40.8
39.2
40.4d
38.9
40.4
38.9
39.4e
100% OM
40.0
39.8
38.7
39.5e
42.2
39.2
39.2
40.2d
Average (H)
41.9b
41.9b
43.5a
42.1 a
41.9b
42.5a
Grain yield (kg/fed)
100 % Mineral
2751.9
2915.6
2694.5
2787.3b
17.4
17.1
38.9
2785.5
2840.3
2712.3
2779.4b
151.3
112.7
ns
75 % M +25% OM
3095.1
3094.1
2725.1
2971.4a
2850.5
3056.8
3020.5
2975.9a
50 M + 50 OM
2438.5
2598.5
2243.5
2426.8c
2827.2
2700.5
2617.2
2715.0b
25 M + 75 OM
2338.5
2078.5
2173.1
2196.7d
2640.5
2487.2
2153.8
2427.2c
100% OM
2285.1
2040.1
1758.5
2027.9e
2377.2
2470.5
2313.8
2387.2c
Average (H)
2581.8a
2545.4b
2318.9c
2696.2a
2711.1a
2563.5b
- Mean values in the same column/row marked with the same letters are not significantly different at 0.05 level of probability.
- ns: not significant difference at 0.05 level of probability according to LSD.
Data in Table (5) reported that the effect of mineral fertilization and organic manure and their interaction was significant on straw yield, biological yield (kg/fed.) and harvest index (HI %) of three maize hybrids in 2015 and 2016 seasons.
The effect of mineral fertilization and organic manure significantly affected the straw yield, biological yield (kg/fed.) and harvest index (HI %) in both seasons, whereas application of 75 % mineral fertilization and 25% organic manure recorded the highest mean values for straw and biological yields while 100% mineral fertilization gave the highest value of HI% in two seasons (Table 5). These findings agreed with Kandil (2004), Siam et al. (2008), Szulc and Hubert (2010), El-Agrodi et al. (2011), Nasim et al. (2012) and Bilal et al.(2016).who showed that yield and its components of maize increased by application of organic fertilizer.
The results in Table (5) stated that the three maize hybrids had significant difference in straw yield, biological yield (kg/fed.) and harvest index (HI %) in both growing seasons. Whereas, the hybrid SC. 2031 recorded the heaviest straw yield, and biological yield (kg/fed.) in both seasons. Meanwhile the highest HI % was given with Sc.2030 in the first season, only. Variations in grain number might be due to differences in genetic potential of maize hybrids. On the other side there was no significant difference among the three maize hybrids in the first season. The obtained results are in confirmed with Kandil (2013) who indicated that there was significant difference among maize hybrids in straw and biological yields as well as HI%.
The findings in Table (5) revealed the significant interaction between the combination of mineral + organic manure on straw yield, biological yield (kg/fed.) and harvest index (HI %). Whereas the highest mean values for straw yield in the second season, were obtained from SC. 2031 when fertilization 25% mineral + 75% organic manure. On the other hand the highest value of biological yield (kg/fed.) and harvest index (HI %) were given with Sc. 2031 + 75% M +25% Om in the first season, only. Meanwhile the lightest straw yield recorded with Sc.203 + 1% OM in the first season, TWC 1100 + 100% organic manure in 2016 season. However the lowest biological yield/fed was obtained with fertilizing Sc.2030 by 100 % organic manure in the first season only. On the other hand there is no significant interaction between two factors on straw yield in the first season, on biological yield/fed in the second season, and on HI % in the first and second seasons.
Table (5). Average of plant attributes for maize hybrids as affected by mineral (M), organic manure (OM) fertilization and their interaction during 2015 and 2016 seasons
Attribute
Fertilizer (F)
Season 2014/2015
Season 2015/2016
Maize hybrid (H)
Average (H)
LSD at 0.05
Maize hybrid (H)
Average (C)
LSD at 0.05
SC.
2031
SC.
2030
TWC
1100
F
H
F x H
SC.
2031
SC.
2030
TWC
1100
F
H
F x H
Straw yield kg/fed.
100 % Mineral
4091.4
3662.0
3983.3
3912.2b
219.1
203.6
ns
3526.5
4391.7
3791.7
3903.3a
198.4
230.9
519.9
75 % M +25% OM
4816.9
4160.6
4234.0
4403.8a
3557.5
3846.4
3795.5
3733.1ab
50 M + 50 OM
3897.6
4121.6
3624.6
3881.3b
3524.8
3347.5
3230.8
3367.7c
25 M + 75 OM
3925.6
3733.2
3757.6
3805.5bc
4219.6
3311.5
3174.5
3568.5b
100% OM
3757.6
3393.6
3526.1
3559.1c
3133.8
3025.5
2806.2
2988.5d
Average (H)
4097.8a
3814.2b
3825.1b
3592.4a
3584.5ab
3359.7b
Biological yield (kg/fed)
100 % Mineral
6843.3
6577.6
6677.8
6699.6b
218.9
203.2
457.7
6312.0
7232.0
6504.0
6682.7a
317.9
258.1
ns
75 % M +25% OM
7912.0
7254.7
6959.1
7375.3a
6408.0
6903.2
6816.0
6709.1a
50 M + 50 OM
6336.1
6720.1
5868.1
6308.1c
6352.0
6048.0
5848.0
6082.7b
25 M + 75 OM
6210.7
5773.3
5516.1
5833.4d
6860.1
5798.7
5328.3
5995.7b
100% OM
6096.1
5472.1
5699.2
5755.8d
5511.0
5496.0
5120.0
5375.7c
Average (H)
6679.6a
6359.6b
6144.1c
6288.6a
6295.6a
5923.3b
Harvest index(HI%)
100 % Mineral
6843.3
6577.6
6677.8
41.6a
1.3
1.2
ns
44.1
39.3
41.7
41.7b
ns
1.9
ns
75 % M +25% OM
7912.0
7254.7
6959.1
40.3b
44.5
44.3
44.3
44.4a
50 M + 50 OM
6336.1
6720.1
5868.1
38.5c
44.5
44.7
44.8
44.6a
25 M + 75 OM
6096.1
5472.1
5699.2
38.2c
38.5
42.9
40.4
40.6b
100% OM
6210.7
5773.3
5516.1
34.7d
43.1
45.0
45.2
44.4a
Average (H)
6679.6b
6359.6a
6144.1b
42.9
43.2
43.3
- Mean values in the same column/row marked with the same letters are not significantly different at 0.05 level of probability.
- ns: not significant difference at 0.05 level of probability according to LSD.
CONCLUSION
It can be concluded that fertilizing maize hybrid Sc. 2031 by 75% mineral fertilization + 25% organic manure achieved the highest values of yield and its components under study conditions.
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